pxl002 ishrna beta catenin 1 (Addgene inc)
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pxl002 ishrna beta catenin 1
Pxl002 Ishrna Beta Catenin 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pxl002/pXL002-ishRNA-beta-catenin-1+(Plasmid+%2336297)/pmc08340846-60-7-8
Average 92 stars, based on 5 article reviews
Pxl002 Ishrna Beta Catenin 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pxl002/pXL002-ishRNA-beta-catenin-1+(Plasmid+%2336297)/pmc08340846-60-7-8
Average 92 stars, based on 5 article reviews
pxl002 ishrna beta catenin 1 - by Bioz Stars,
2026-10
92/100 stars
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Transfection:Article Title: Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions. Article Snippet: BSA (Sigma, cat. no. A9418) CHIR99021 (Selleckchem, cat. no. S1263-25 mg) (Steps 12A and 12B only) DMEM (Life Technologies, cat. no. 11965-092) DMEM/F12 (Life Technologies, cat. no. 11330-057) DMSO (Sigma, cat. no. D8418) Doxycycline (Stemgent, cat. no. 04-0016) (Step 12A only) • • • • • • • • • • • • • • • • • FBS (Life Technologies, cat. no. 16000-044) FuGENE HD transfection reagent (Promega, cat. no. E2311) Gelatin (type A, porcine skin) (Sigma, cat. no. G1890) Gold antifade reagent with DAPI (Life Technologies, cat. no. P-36931) GlutaMAX (Life Technologies, cat. no. 35050-061) Hydrochloric acid (Sigma, cat. no. H1758) Human pluripotent stem cell lines, e.g., 19-9-11, 6-9-9, IMR90C4, H9, H13, H14 (WISC Bank) IWP2 (Tocris, cat. no. 3533-10 mg) (Step 12B only) IWP4 (Stemgent, cat. no. 04-0036) (Step 12B only) KnockOut serum replacement (Life Technologies, cat. no. 10828-028) l-Glutamine (Life Technologies, cat. no. 25030) RPMI (Life Technologies, cat. no. 11875-119) mTeSR1 (STEMCELL Technologies, cat. no. 05857) Matrigel (BD Biosciences, cat. no. 354277) MEM non-essential amino acid (Life Technologies, cat. no. 11140) (Step 12A only) Nonfat dry milk (Bio-Rad, cat. no. 170-6404XTU) One Shot Stbl3 chemically competent Escherichia coli (Life Technologies, cat. no. C7373-03) (Step 12A only) Omniscript reverse transcription (RT) kit (Qiagen, cat. no. 205111) (Step 12A only) Opti-MEM (Life Technologies, cat. no. 51985-034) (Step 12A only) PureLink HiPure plasmid midiprep kit (Life Technologies, cat. no. K2100-04) (Step 12A only) Knock-Out:Article Title: Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions. Article Snippet: BSA (Sigma, cat. no. A9418) CHIR99021 (Selleckchem, cat. no. S1263-25 mg) (Steps 12A and 12B only) DMEM (Life Technologies, cat. no. 11965-092) DMEM/F12 (Life Technologies, cat. no. 11330-057) DMSO (Sigma, cat. no. D8418) Doxycycline (Stemgent, cat. no. 04-0016) (Step 12A only) • • • • • • • • • • • • • • • • • FBS (Life Technologies, cat. no. 16000-044) FuGENE HD transfection reagent (Promega, cat. no. E2311) Gelatin (type A, porcine skin) (Sigma, cat. no. G1890) Gold antifade reagent with DAPI (Life Technologies, cat. no. P-36931) GlutaMAX (Life Technologies, cat. no. 35050-061) Hydrochloric acid (Sigma, cat. no. H1758) Human pluripotent stem cell lines, e.g., 19-9-11, 6-9-9, IMR90C4, H9, H13, H14 (WISC Bank) IWP2 (Tocris, cat. no. 3533-10 mg) (Step 12B only) IWP4 (Stemgent, cat. no. 04-0036) (Step 12B only) KnockOut serum replacement (Life Technologies, cat. no. 10828-028) l-Glutamine (Life Technologies, cat. no. 25030) RPMI (Life Technologies, cat. no. 11875-119) mTeSR1 (STEMCELL Technologies, cat. no. 05857) Matrigel (BD Biosciences, cat. no. 354277) MEM non-essential amino acid (Life Technologies, cat. no. 11140) (Step 12A only) Nonfat dry milk (Bio-Rad, cat. no. 170-6404XTU) One Shot Stbl3 chemically competent Escherichia coli (Life Technologies, cat. no. C7373-03) (Step 12A only) Omniscript reverse transcription (RT) kit (Qiagen, cat. no. 205111) (Step 12A only) Opti-MEM (Life Technologies, cat. no. 51985-034) (Step 12A only) PureLink HiPure plasmid midiprep kit (Life Technologies, cat. no. K2100-04) (Step 12A only) Reverse Transcription:Article Title: Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions. Article Snippet: BSA (Sigma, cat. no. A9418) CHIR99021 (Selleckchem, cat. no. S1263-25 mg) (Steps 12A and 12B only) DMEM (Life Technologies, cat. no. 11965-092) DMEM/F12 (Life Technologies, cat. no. 11330-057) DMSO (Sigma, cat. no. D8418) Doxycycline (Stemgent, cat. no. 04-0016) (Step 12A only) • • • • • • • • • • • • • • • • • FBS (Life Technologies, cat. no. 16000-044) FuGENE HD transfection reagent (Promega, cat. no. E2311) Gelatin (type A, porcine skin) (Sigma, cat. no. G1890) Gold antifade reagent with DAPI (Life Technologies, cat. no. P-36931) GlutaMAX (Life Technologies, cat. no. 35050-061) Hydrochloric acid (Sigma, cat. no. H1758) Human pluripotent stem cell lines, e.g., 19-9-11, 6-9-9, IMR90C4, H9, H13, H14 (WISC Bank) IWP2 (Tocris, cat. no. 3533-10 mg) (Step 12B only) IWP4 (Stemgent, cat. no. 04-0036) (Step 12B only) KnockOut serum replacement (Life Technologies, cat. no. 10828-028) l-Glutamine (Life Technologies, cat. no. 25030) RPMI (Life Technologies, cat. no. 11875-119) mTeSR1 (STEMCELL Technologies, cat. no. 05857) Matrigel (BD Biosciences, cat. no. 354277) MEM non-essential amino acid (Life Technologies, cat. no. 11140) (Step 12A only) Nonfat dry milk (Bio-Rad, cat. no. 170-6404XTU) One Shot Stbl3 chemically competent Escherichia coli (Life Technologies, cat. no. C7373-03) (Step 12A only) Omniscript reverse transcription (RT) kit (Qiagen, cat. no. 205111) (Step 12A only) Opti-MEM (Life Technologies, cat. no. 51985-034) (Step 12A only) PureLink HiPure plasmid midiprep kit (Life Technologies, cat. no. K2100-04) (Step 12A only) Plasmid Preparation:Article Title: Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions. Article Snippet: BSA (Sigma, cat. no. A9418) CHIR99021 (Selleckchem, cat. no. S1263-25 mg) (Steps 12A and 12B only) DMEM (Life Technologies, cat. no. 11965-092) DMEM/F12 (Life Technologies, cat. no. 11330-057) DMSO (Sigma, cat. no. D8418) Doxycycline (Stemgent, cat. no. 04-0016) (Step 12A only) • • • • • • • • • • • • • • • • • FBS (Life Technologies, cat. no. 16000-044) FuGENE HD transfection reagent (Promega, cat. no. E2311) Gelatin (type A, porcine skin) (Sigma, cat. no. G1890) Gold antifade reagent with DAPI (Life Technologies, cat. no. P-36931) GlutaMAX (Life Technologies, cat. no. 35050-061) Hydrochloric acid (Sigma, cat. no. H1758) Human pluripotent stem cell lines, e.g., 19-9-11, 6-9-9, IMR90C4, H9, H13, H14 (WISC Bank) IWP2 (Tocris, cat. no. 3533-10 mg) (Step 12B only) IWP4 (Stemgent, cat. no. 04-0036) (Step 12B only) KnockOut serum replacement (Life Technologies, cat. no. 10828-028) l-Glutamine (Life Technologies, cat. no. 25030) RPMI (Life Technologies, cat. no. 11875-119) mTeSR1 (STEMCELL Technologies, cat. no. 05857) Matrigel (BD Biosciences, cat. no. 354277) MEM non-essential amino acid (Life Technologies, cat. no. 11140) (Step 12A only) Nonfat dry milk (Bio-Rad, cat. no. 170-6404XTU) One Shot Stbl3 chemically competent Escherichia coli (Life Technologies, cat. no. C7373-03) (Step 12A only) Omniscript reverse transcription (RT) kit (Qiagen, cat. no. 205111) (Step 12A only) Opti-MEM (Life Technologies, cat. no. 51985-034) (Step 12A only) PureLink HiPure plasmid midiprep kit (Life Technologies, cat. no. K2100-04) (Step 12A only) SYBR Green Assay:Article Title: Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions. Article Snippet: BSA (Sigma, cat. no. A9418) CHIR99021 (Selleckchem, cat. no. S1263-25 mg) (Steps 12A and 12B only) DMEM (Life Technologies, cat. no. 11965-092) DMEM/F12 (Life Technologies, cat. no. 11330-057) DMSO (Sigma, cat. no. D8418) Doxycycline (Stemgent, cat. no. 04-0016) (Step 12A only) • • • • • • • • • • • • • • • • • FBS (Life Technologies, cat. no. 16000-044) FuGENE HD transfection reagent (Promega, cat. no. E2311) Gelatin (type A, porcine skin) (Sigma, cat. no. G1890) Gold antifade reagent with DAPI (Life Technologies, cat. no. P-36931) GlutaMAX (Life Technologies, cat. no. 35050-061) Hydrochloric acid (Sigma, cat. no. H1758) Human pluripotent stem cell lines, e.g., 19-9-11, 6-9-9, IMR90C4, H9, H13, H14 (WISC Bank) IWP2 (Tocris, cat. no. 3533-10 mg) (Step 12B only) IWP4 (Stemgent, cat. no. 04-0036) (Step 12B only) KnockOut serum replacement (Life Technologies, cat. no. 10828-028) l-Glutamine (Life Technologies, cat. no. 25030) RPMI (Life Technologies, cat. no. 11875-119) mTeSR1 (STEMCELL Technologies, cat. no. 05857) Matrigel (BD Biosciences, cat. no. 354277) MEM non-essential amino acid (Life Technologies, cat. no. 11140) (Step 12A only) Nonfat dry milk (Bio-Rad, cat. no. 170-6404XTU) One Shot Stbl3 chemically competent Escherichia coli (Life Technologies, cat. no. C7373-03) (Step 12A only) Omniscript reverse transcription (RT) kit (Qiagen, cat. no. 205111) (Step 12A only) Opti-MEM (Life Technologies, cat. no. 51985-034) (Step 12A only) PureLink HiPure plasmid midiprep kit (Life Technologies, cat. no. K2100-04) (Step 12A only) Polymerase Chain Reaction:Article Title: Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions. Article Snippet: BSA (Sigma, cat. no. A9418) CHIR99021 (Selleckchem, cat. no. S1263-25 mg) (Steps 12A and 12B only) DMEM (Life Technologies, cat. no. 11965-092) DMEM/F12 (Life Technologies, cat. no. 11330-057) DMSO (Sigma, cat. no. D8418) Doxycycline (Stemgent, cat. no. 04-0016) (Step 12A only) • • • • • • • • • • • • • • • • • FBS (Life Technologies, cat. no. 16000-044) FuGENE HD transfection reagent (Promega, cat. no. E2311) Gelatin (type A, porcine skin) (Sigma, cat. no. G1890) Gold antifade reagent with DAPI (Life Technologies, cat. no. P-36931) GlutaMAX (Life Technologies, cat. no. 35050-061) Hydrochloric acid (Sigma, cat. no. H1758) Human pluripotent stem cell lines, e.g., 19-9-11, 6-9-9, IMR90C4, H9, H13, H14 (WISC Bank) IWP2 (Tocris, cat. no. 3533-10 mg) (Step 12B only) IWP4 (Stemgent, cat. no. 04-0036) (Step 12B only) KnockOut serum replacement (Life Technologies, cat. no. 10828-028) l-Glutamine (Life Technologies, cat. no. 25030) RPMI (Life Technologies, cat. no. 11875-119) mTeSR1 (STEMCELL Technologies, cat. no. 05857) Matrigel (BD Biosciences, cat. no. 354277) MEM non-essential amino acid (Life Technologies, cat. no. 11140) (Step 12A only) Nonfat dry milk (Bio-Rad, cat. no. 170-6404XTU) One Shot Stbl3 chemically competent Escherichia coli (Life Technologies, cat. no. C7373-03) (Step 12A only) Omniscript reverse transcription (RT) kit (Qiagen, cat. no. 205111) (Step 12A only) Opti-MEM (Life Technologies, cat. no. 51985-034) (Step 12A only) PureLink HiPure plasmid midiprep kit (Life Technologies, cat. no. K2100-04) (Step 12A only) Quantitative RT-PCR:Article Title: Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions. Article Snippet: BSA (Sigma, cat. no. A9418) CHIR99021 (Selleckchem, cat. no. S1263-25 mg) (Steps 12A and 12B only) DMEM (Life Technologies, cat. no. 11965-092) DMEM/F12 (Life Technologies, cat. no. 11330-057) DMSO (Sigma, cat. no. D8418) Doxycycline (Stemgent, cat. no. 04-0016) (Step 12A only) • • • • • • • • • • • • • • • • • FBS (Life Technologies, cat. no. 16000-044) FuGENE HD transfection reagent (Promega, cat. no. E2311) Gelatin (type A, porcine skin) (Sigma, cat. no. G1890) Gold antifade reagent with DAPI (Life Technologies, cat. no. P-36931) GlutaMAX (Life Technologies, cat. no. 35050-061) Hydrochloric acid (Sigma, cat. no. H1758) Human pluripotent stem cell lines, e.g., 19-9-11, 6-9-9, IMR90C4, H9, H13, H14 (WISC Bank) IWP2 (Tocris, cat. no. 3533-10 mg) (Step 12B only) IWP4 (Stemgent, cat. no. 04-0036) (Step 12B only) KnockOut serum replacement (Life Technologies, cat. no. 10828-028) l-Glutamine (Life Technologies, cat. no. 25030) RPMI (Life Technologies, cat. no. 11875-119) mTeSR1 (STEMCELL Technologies, cat. no. 05857) Matrigel (BD Biosciences, cat. no. 354277) MEM non-essential amino acid (Life Technologies, cat. no. 11140) (Step 12A only) Nonfat dry milk (Bio-Rad, cat. no. 170-6404XTU) One Shot Stbl3 chemically competent Escherichia coli (Life Technologies, cat. no. C7373-03) (Step 12A only) Omniscript reverse transcription (RT) kit (Qiagen, cat. no. 205111) (Step 12A only) Opti-MEM (Life Technologies, cat. no. 51985-034) (Step 12A only) PureLink HiPure plasmid midiprep kit (Life Technologies, cat. no. K2100-04) (Step 12A only) |